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Because NNMT activity intersects methyl donor utilization and nicotinamide handling, experimental inhibition can be used to probe pathway level relationships among methyl economy balance, NAD+ salvage flux, and downstream metabolic signaling in a range of preclinical systems

Escitalopram (Lundbeck, Denmark) was dissolved in saline and was administered intraperitoneally daily in a dosage of 5 mg/kg

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For instance, the MitoSOX Red probe (Invitrogen, Waltham, MA, USA) can be used to measure oxidative stress localized in the mitochondria, whereas the CellROX Green (Invitrogen, Waltham, MA, USA) and Deep Red probes (Invitrogen, Waltham, MA, USA) can be used to detect intracellular ROS