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After binding for 10 min at 37C, the binding complexes were separated by centrifugation (12,000 g for 10 min) and concentrations of bound and free peptides were determined by using the Lowry method [29]

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Butcher SK, Chahal H, Nayak L, Sinclair A, Henriquez NV, Sapey E, et al

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Marcus sees herself taking the pill long term, with a goal of losing around 30 pounds

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