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PBMCs were prepared using Ficoll-Paque Plus (Cytiva) by the standard protocol and T cell was isolated with PanT cell Isolation kit (Miltenyi Biotec) by the manufacturer instruction

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On the practical question, the evidence points one way: combine at the point of use if at all one syringe or two, given close together and keep them as separate vials in storage, where there is no stability data to justify a pre-mixed blend

Mol Cell Proteomics 3 , 501509, doi: 10.1074/mcp.M300132-MCP200 (2004)