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A NanoBRET membrane competition binding assay was employed to assess the ability of each peptide to inhibit binding of the fluorescent probe ROX-Ex4 to the GLP-1R N-terminally tagged with nanoluciferase (Nluc)

Pulmonary ILC2 isolation and ex vivo experiments Mice were intranasally challenged with 0.5 g of rmIL-33 (BioLegend) once per day for three days before tissue collection

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There is no established pharmacological mechanism by which semaglutide would directly cause stool discolouration

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