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However, the underlying mechanisms of pancreatic islet fibrosis and -cell failure remain unclear

10.1016/S2213-2600(19)30374-1 124 MaJ

What is the standard protocol

So I took a break for a week and came back later with renewed forces and with more patience

The cells were cultured in Eagles minimum essential medium (ThermoFisher Scientific) or DMEM F-12 (ThermoFisher Scientific) respectively, supplemented with 10% Fetal Bovine Serum (Gibco) and 1% penicillinstreptomycin (ThermoFisher Scientific), in a humidified incubator maintaining 5% CO 2 at 37 C

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