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Pen Dosage Chart Dosage & Protocols Variations Standard Research Protocol Dose: 0.3 1.2 mg (= 1248 clicks) Duration: 8 12 weeks Frequency: Once weekly Cycle Interval: 4 weeks off before repeating Goal / Description: Baseline protocol for appetite regulation and body weight modulation in experimental models

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The GAPDH gene was served as the internal control ( CT method ( Cloning, expression and identification of rSmGST The first Spirometra GST gene deposited in GeneBank (AEI16476.1) was amplified by PCR with specific primers carrying BamHI and PstI restriction enzyme sites (underlined) (forward, 5-AT GGATCC ATGGGTTC GCTCCCGGTTC-3, and reverse, 5-AT CTGCAG CTAAGCATCACCACGCCAG-3), and the cycling protocol was as follows: 30 cycles of 95C for 50 s, 60C for 50 s and 72C for 50 s

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