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For the preparation of nuclear extracts, frozen liver pieces were homogenized in homogenization buffer (20 mM HEPES, 70 mM sucrose, 220 mM Mannitol, 2 mM EDTA, 0.5 mg/ml BSA, 0.1 mM PMSF, 1.0 mM DTT, pH 7.5)

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Excessive feeding can cause metabolic dysfunction (109), as well as altered peptide signaling for appetite and satiety, including YY (101), GLP-1, GIP, and CCK (109)

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