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Reconstitution A: 10 mg vial + 2 mL BAC water (5 mg/mL) the standard "20 units = 1 mg" dilution

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After filtration and centrifugation, the cell pellet was resuspended with low glucose Dulbeccos modified Eagles medium (DMEM, Gibco) containing 10% fetal bovine serum (FBS, Gibco) and 1% penicillinstreptomycin (Gibco) at 37 C and 5% CO2 in 10-cm dishes