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3D-Spheroid assay 96-well plates were coated with 50 L of 1.5 % sterile agarose 2 h before cell plating

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Use a moisturiser containing ingredients like hyaluronic acid [1] or ceramides

Regular follow-up appointments will ensure that the treatment is working effectively and allow any necessary adjustments to be made

Growth differentiation factor-15 promotes glutamate release in medial prefrontal cortex of mice through upregulation of T-type calcium channels

Stronger mechanistic clarification would require isoform-specific NOS inhibition, direct measurement of NO metabolites, eNOS phosphorylation analysis, rescue experiments, and comparison across endothelial, epithelial, fibroblast, and neuronal model systems