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Samples were fixed in 10% buffered formalin overnight at 4C, dehydrated with increasing concentrations of ethanol, embedded in paraffin, cut into 5 m sections, and stained with hematoxylin and eosin (HE) or Massons Trichrome Stain Kit (Sigma-Aldrich)

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Amyotrophic lateral sclerosis, ALS Amyotrophic lateral sclerosis is a malignant and rapid degeneration of upper and lower motor neurones located in the cortex, brain stem and spinal cord