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Suspend the mince in Solution B and dissociate the tissue by one of the following methods: (a) 500 g papain per brain for 20 min at room temperature followed by DNase I (1 mg/ml) at 37C for 5 min [1] OR (b) 0.25% trypsin and 0.1 mg/ml DNase I for 20 min at 37C [2]
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Interestingly and similarly, differences in energy metabolism, such as glycerophospholipid metabolism, citrate cycle, and alanine, aspartate, and glutamate metabolism, were also observed in serum and urine between normal and CAG rats in these studies [85,86]
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These evidence stems from epidemiological, histopathological and molecular pathological studies (Ames et al., 1995
