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Plasmids of wildtype and mutants of ARE motifs were transfected to K562 (#CRL-3344, ATCC), an erythroleukemia cell, along with pDsRed1-N1 (PT3405, Clontech), which is used as a transfection efficacy control

If GSH is depleted due to oxidative stress, inflammation, or exposure to xenobiotics, de novo synthesis of GSH is upregulated primarily by increasing availability of cysteine through recycling of GSSG

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10.1038/s41392-021-00679-0 Signal Transduct Target Ther

[1] The results were exciting: [1] While L-citrulline alone increased plasma arginine, the combination with glutathione was most effective at increasing plasma nitrite and nitric oxide metabolites (NOx)

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