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(These events are not well-reported for bacteriostatic water specifically, but benzyl alcohol hypersensitivity can occur.) Hemolysis (red blood cell destruction): Important: Bacteriostatic water is a hypotonic solution
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Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]
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