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The levels of protein-bound glutathione in patients in the current study were considerably higher than those of healthy volunteers in previous studies [12] estimated using the same method

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In simple terms, it "weighs" the peptide

Metabolome analysis by LC-MS/MS 3 10 6 million cells were washed in 1 ml of PBS, pelleted at 1500 rcf in an Eppendorf centrifuge at 4 o C, and resuspended in 100 l of 80% methanol (80 C) and 10 l of 0.3 mM 5-thio-glucose, used as internal standard to allow correction for sample recovery