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Prioritize non-starchy vegetables like spinach, broccoli, bell peppers, zucchini, and green beans, which deliver vitamins and minerals without excess calories

NG239 Evidence review D: tests for identifying cause of vitamin B12 deficiency

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For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)

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Its dosing schedule is designed to gradually increase from 0.25 mg to a maximum of 2.4 mg per week over 1620 weeks