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Combining the changes in the three groups, at the genus level, Clostridium_sensu_stricto_1 was increased in the TS group compared to the CON group, and GABA treatment alleviated the Clostridium_sensu_stricto_1 abnormality, suggesting a potential mechanism (Figure 2D)

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Although the meta-regression did not significantly reduce the overall heterogeneity, it confirms that the high heterogeneity is driven by a combination of multiple factors rather than a single dominant study feature

Serious adverse events Beyond GI discomfort, higher semaglutide doses carry documented risks for specific serious events

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Cells were harvested by centrifugation and washed in M9 minimal medium