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Human and macaque tissue sections were pretreated for 30 min with HIER at 95 C in ER2 (Leica) followed by a 15-min protease treatment and then hybridized with human-specific probes to detect mRNA transcripts for CALCR (483048), PRLH (526568-C2) and GLP1R (519828- C3

This means that ferroptosis can induce other kinds of cell death, and/or it plays a minor effect in cell death, or we use inhibitors after the reversible point and once this threshold is exceeded, ferroptosis will be irreversible Recently, we also found that ferroptosis is vital for tumor immunity such as macrophage phagocytosis (71) and T cell killing (115) but the crosstalk between ferroptosis and the glioma immunosuppressive microenvironment is not clear

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