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While nutritional VC intake demonstrates chemopreventive effects by scavenging carcinogen-induced reactive oxygen species (ROS) and maintaining genomic stability, high-dose intravenous VC acts as a prooxidant to selectively kill tumor cells via ROS-mediated deoxyribonucleic acid (DNA) damage, adenosine triphosphate (ATP) depletion, and HIF-1 degradation
PubMed , International Archives of Occupational and Environmental Health , vol
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After one hour of incubation at RT in the dark, 1 ml of 20% (w/v) TCA was added to the samples