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Chemicals for MALDI-TOF/MS 2,5-Dihydroxybenzoic acid (DHB), -cyano-4-hydroxycinnamic acid (-HCCA), methanol, acetonitrile, 1 mM ammonium acetate, 0.1 % TFA, and 50 % sulfosalicylic acid (SSA) were obtained from Sigma-Aldrich

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The downstream effects include: Synaptogenesis : Formation of new synaptic connections in hippocampal neurons, driven by c-Met-mediated activation of PI3K/AKT and MAPK/ERK signaling cascades Spinogenesis : Growth of new dendritic spines, the structural substrate for excitatory synaptic transmission Neurotrophic support : In cell-based neurotrophic assays, Dihexa demonstrated potency approximately seven orders of magnitude greater than BDNF The HGF/c-Met pathway is distinct from the BDNF/TrkB and NGF/TrkA pathways targeted by most nootropic peptides

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44-46 High blood glucose levels trigger the production of large amounts of advanced glycation end-products (AGEs), proteins and lipids damaged by spontaneous reactions with glucose