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The flow rate for each run started 100% B for 2 min at 0.2 ml/min, followed by a gradient starting at 20% A/80% B changing linearly to 70% A/30% B over the course of 1 min at 0.7 ml/min, followed by 70% A/30% B for 5 min at 0.7 ml/min, and finally to 100% B over a course of 5 min

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These amounts are dependent upon the percentage of alcohol by volume and many beers, wines, and spirits do not follow this standard

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Anaesthesia 2000

While high-dose oral B12 (1,000 mcg daily) may be effective in some cases, intramuscular hydroxocobalamin remains the standard NHS practice