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Increased ROS levels in human and murine primary RPE [43] and in ARPE-19 cells [44] cultured under HG conditions have been reported

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NMR studies were performed in NMR buffer 100 mM Tris pH 8.0, 100 mM NaCl, 2 mM DTT, 50 M DSS, 0.02% sodium azide, and 5% D 2 O) and 1% DMSO across all concentration points

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