Sample volumes were normalized to 35 L with EPPS buffer (200 mM, pH 8), diluted with HPLC grade CH 3 CN (9 L), and then labeled (5 L of 20 g/L per sample) with the corresponding TMTsixplex Isobaric Mass Tag (Themo Scientific, #90064B)
In more severe cases, surgery may be needed to repair the tears
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25,35 However, as Glu resembles Asp, using Asp 15 from GIP in tirzepatide should not influence its GLP-1 activity