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HUVECs and NIH 3T3 cells in Roswell Park Memorial Institute (RPMI) 1640 and HaCaT in Dulbeccos Minimum Essential Medium (DMEM)/F-12 medium were cultured in the indicated media supplemented with 10% fetal bovine serum (FBS) and maintained at 37C in a humidified environment with 5% CO 2

Cells were subjected to treatment in incubator for 24 hr

Furthermore, these approaches, like levodopa administration or deep brain stimulation, have no capacity to restore lost function

This approach helps reduce degradation during shipping and allows the material to maintain its quality under appropriate storage conditions

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