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In vitro ubiquitylation assays All ubiquitylation assays were performed at room temperature in the final reaction buffer containing 25 mM HEPES pH 7.5, 150 mM NaCl, 0.25 mM DTT, 5 mM ATP and 10 mM MgCl 2

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Reduction of Free Radicals: Glutathione neutralizes free radicals and oxidative stress, which can trigger increased melanin production

Animal Research Animal research contributes to Retatrutides broader evidence landscape by allowing controlled exploration of pathway-level questions that cannot be fully isolated in human trials

About the Authors Medically reviewed by Phyllis J