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Similarly, the transwell chamber assay corroborated these findings, demonstrating that overexpression of SLC25A39 markedly increased CRC cell migration, while knockdown of SLC25A39 had the opposite effect (Fig

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In contrast, cells with lower senescence burden may have less FOXO4p53 dependence, or may buffer p53 relocalization through other regulators, which might explain the limited apoptosis in younger cultures compared to aged cell cultures