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doi: 10.1530/EJE-16-0517

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Immunoprecipitation To enrich PDHc-E2 or OGDHc-E2 for proteomics analysis, 5 10 6 cells were lysed in 1.5 ml IP buffer (50 mM HEPES (pH 7.0), 150 mM NaCl, 1% (vol/vol) IGEPAL CA-630, 1 mM phenylmethylsulfonyl fluoride, 10 mM Tris(2-carboxyethyl)phosphine hydrochloride, 100 mM nicotinamide, 1 Roche Complete Protease Inhibitor Cocktail) and rotated at 4 C for 30 min

We plan to achieve this goal by efficiently advancing our portfolio of GPCR-targeted programs, continuing to expand our differentiated GPCR-targeted pipeline focused on indications with significant unmet needs, maximizing the potential of our Native Complex Platform through continued innovation and investment, and evaluating potential value-creating strategic partnerships

Elevated Levels of Cholesterol-Rich Lipid Rafts in Cancer Cells Are Correlated with Apoptosis Sensitivity Induced by Cholesterol-Depleting Agents