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*JAMA*, 281(15), 1415-1423

Cells were permeabilized with 0.5% Triton-X 100 in PBS for 5 min at room temperature, then the permeabilization buffer was removed, and cells were washed twice with 0.1% Tween-20 in PBS

In eye diseases, it prevents the interaction of VEGF with its receptors on the surface of endothelial cells, reducing endothelial cell proliferation, vascular leakage and the formation of new blood vessels in the retina

The copper chelation appears to facilitate cellular uptake through copper transport proteins expressed in keratinocytes and fibroblasts

Additional information Competing interests The authors declare that they have no competing interests