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Additionally, the interaction between substance abuse and depression may worsen depressive symptoms and its prognosis, leading to a vicious cycle that further complicates metabolic health [48]

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Recent emerging studies, such as the investigation into Aconitum pendulum polysaccharides (APPs), have explicitly recognized this limitation, stating that while microbial shifts were observed, causal verification via FMT remains essential for future validation ( in vivo blocking), thus forming the shortest closed-loop evidence chain of key molecules-key receptors/pathways-key phenotypes

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Always follow your laboratory's protocol or the manufacturer's documentation when determining reconstitution volumes

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