Methods Human cell culture and lysis Human epithelial cervix carcinoma HeLa cells (female) and human retinal pigment epithelial RPE1 cells (female) immortalized with hTERT were purchased from ATCC (CCL-2 and CRL-4002, respectively) and cultured in DMEM (Gibco, Invitrogen) supplemented with 10% fetal bovine serum (Gibco, Invitrogen), 100 U/ml penicillin (Gibco, Invitrogen), 100 g/ml streptomycin (Gibco, Invitrogen) at 37 C in a humidified incubator with 5% CO 2
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Thus, alterations in microbiome diversity may initially trigger the development of HFpEF and after onset of the disease intestinal damage might be accelerated in a vicious cycle
Methodological strengths of present study for supporting analytic framework of future real-world CEAs The present CEA has several methodological strengths
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