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Louis, MO, USA), or sterilized distilled water (2.5 l) as a negative control

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However, not enough is known about this theoretical issue to elucidate a risk-benefit tradeoff and how timing of treatment works into such a tradeoff

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After three 20-min washes in 1 TBS at 4 C, the peptide-bound XPO1 was transferred onto a PVDF membrane using a semi-dry blotting system (1.0 mA/cm, 4 C, 30 min)