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Then, the dishes were washed by PBS, fixed with 4% paraformaldehyde, permeabilized by 0.2% Triton X-PBS solution, and then incubated with primary antibodies (p-P38, p-P65, KEAP1, Ubiquitin, and LC3) at 4 C overnight

Verbal consent was obtained when there were limitations with literacy and the individual expressed a general disinclination to signing written documents that cannot be read and fully comprehended by them

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