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Introduction The technology of semen cryopreservation effectively promotes the application of technologies including genetic resource conservation, zootechnical management, and artificial insemination ( However, the cold shock and alterations in atmospheric oxygen seriously led to biochemical, biophysical, ultrastructural, molecular, and functional alterations ( To ameliorate oxidative stress-related damage, the supplementations of antioxidants, including vitamins, coenzymes, exosomes, liposomes, nanomaterials and trace elements such as selenium (Se), copper, and zinc to semen extender have been widely conducted (29)

doi: 10.1016/j.cmet.2009.02.002

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