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Next, we investigated effect of freeze-thaw cycle on blood NADs and glutathione concentration

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In the current study, we applied a porous cellulose sponge as an immobilization carrier, because its cellulose fibers were thought to be molded more stably and had greater mechanical strength than the filter paper

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Due to its strong affinity for copper ions, GHK-Cu serves as a well-established model molecule for analyzing metal-dependent cellular processes