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A serum B12 level test and ideally a methylmalonic acid test should be performed before starting B12 injections

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Compounded medications are not FDA-approved but are prepared in compliance with Section 503A of the Federal Food, Drug, and Cosmetic Act

Check the FDA's 503B outsourcing-facility list to see whether a cGMP-compliant alternative exists

DAB staining and preparation of cultured cells for EM The fixative was removed and cells were washed (five times for 1 min) with 0.1 M sodium cacodylate (Ted Pella, 18851) buffer at pH 7.4 containing 2 mM CaCl 2 on ice and blocked with 20 mM glycine in 0.1 M sodium cacodylate buffer pH 7.4 containing 2 mM CaCl 2 for 20 min on ice