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For the GSSG estimation, 100 L of the supernatant was mixed with 900 L of reaction buffer containing NADPH (nicotinamide adenine dinucleotide phosphate, reduced form, 0.3 mM) and GR (30 U mL 1 ) and incubated at room temperature for 30 min

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The key enzymes in the biosynthesis of glutathione are the cytosolic glutamate-cysteine-ligase (GCL