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J Hepatol (2019) 70:15171

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Plasmid constructions and reporter activity assay The 1200-bp DNA fragment containing the CBS promoter region from 1000 to +200 bp was amplified from SKOV3 Era-R cell genomic DNA by PCR with the following primers: CBS- Kpn I forward, 5-GGTACCGACATTTAATTCTAATTCACGTCTC-3 and CBS- Bgl II reverse, 5-AGATCTGTCCAGAGAGGGGAGCGAGTCTCGG-3

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