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Then, we placed the soluble lysates into a tumbler at 4 C with GSH-Sepharose 4B or Ni-NTA resin (GE Healthcare)

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For instance, often times a renal transporter is referred to as being involved in secretion of an ion or solute if it is found in the basolateral membrane when in fact its function may simply be the uptake, from the blood, into the cell of the ion or solute