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Then, the reaction mixtures were diluted with adequate amounts of 5 mM sodium phosphate buffer (pH 7.4) containing 10% (v/v) acetonitrile and filtered using a 0.45-m filter (Cosmonice filter W, Nacalai Tesque)

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Tandem cleavable linkers prodrug platform utilizing Val-Cit linkage chemistry has been reported by Su et al