This article describes longitudinal tracking of lung function from birth to young adult life
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Open the syringe and needle packets then snuggly fasten the needle onto the end of the syringe
Dissociated cells were suspended in L-15 medium (Liebovitz, L1518, Merck) containing 10% fetal bovine serum, NaHCO 3 , glucose, penicillin/streptomycin (1) and floxuridine (PHR2589, Merck) and plated on coverslips precoated with poly-d-lysine (A-003-E, Merck) and laminin (L2020, Merck)
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