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Corroborating these observations, immunofluorescence staining and flow cytometry analyses further confirmed reduced expression of F4/80 and the M1 macrophage marker CD11c, along with fewer CD11c macrophages in eWAT of HFD-fed Ssu72 aKO mice (Supplementary Fig

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Next, the level of Nrf2 in the nucleus was measured, and our results revealed that GHK-Cu markedly increased its expression (Figures 4B,F)

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As a result, I feel the optimal dosing range for future clinical trials would be 250-500mcg daily