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The peptide was originally characterized by the Sikiric group at the University of Zagreb School of Medicine and has been investigated for over two decades in preclinical research models for its effects on tissue regeneration, angiogenesis, fibroblast migration, and cellular signaling across tendon, ligament, muscle, gastrointestinal, and vascular tissues

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An intervention study in 57 healthy adults who were fed choline-deficient diets under controlled conditions found that 77% of men, 80% of postmenopausal women, and 44% of premenopausal women developed fatty liver, liver damage, and/or muscle damage (17)