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To ascertain the observed signal is not from fatty acids uptake, but from the newly synthesized lipids after metabolic process, we chemically interrogate the lipid metabolic pathway by treating neuroglioma cells with the small molecule inhibitor Triacsin C

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Since the pathways and carbon sources supplying acetyl-CoA in the DKO cells were unknown, DKO cells were cultured in the presence of deuterated water and deuterium incorporation into palmitate was used to examine total de novo lipogenesis

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Yang MT, Chou IC, Wang HS

Attenuation of bleomycin-induced pulmonary fibrosis in rats with S-allyl cysteine