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In a different aliquot, the same extract was initially added with 200 mM phosphate buffer (pH 7.4) and 200 L 10 mM dithiothreitol (DTT) to reduce all dehydroascorbate (DHA) into AsA and continued the same reaction as mentioned

4CL: 4-Coumarate-CoA ligase

Study two In a 1997 study, 11 men aged 64-76 received nightly injections of 2mg sermorelin for six weeks