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We incubated chilled BMDMs with biotin-25HC (at 4 C) to promote 25HC interaction with cell surface moieties in the absence of their internalization

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Wehkamp J, Harder J, Wehkamp K, Wehkamp-von Meissner B, Schlee M, Enders C, Sonnenborn U, Nuding S, Bengmark S, Fellermann K, Schrder JM, Stange EF: NF-B- and AP-1-mediated induction of human -defensin-2 in intestinal epithelial cells by Escherichia coli Nissle 1917: a novel effect of a probiotic bacterium