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Biochem., 77, 464-469 11

Western blot analysis RIPA Lysis Buffer (TransGen, Beijing, China) was used to extract total protein from tissues/fibroblasts and a Nuclear and Cytoplasmic Protein Extraction Kit (TransGen) was used to extract the protein from nucleus and cytoplasm of fibroblasts

Water is constantly on the move

Kazemeini is with the Kirk Kerkorian School of Medicine at UNLV in Las Vegas, Nevada

It is widely used in regenerative settings because it supports tissue repair across multiple systems, particularly connective tissue

For future batches, verify bacteriostatic water sterility by inspecting for clarity and checking the expiration date, use a fresh alcohol swab on the vial stopper before every needle insertion, and never reuse needles or syringes across multiple draws