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Elevated H 2 O 2 production by SOD activity leads to an early inhibition of EAS enzymes activity such as TrxR (Schallreuter et al., 1991a), CAT and GPx, which in turn, leads to an increase in Fenton reaction, increasing hydroxyl concentrations (Fe 2+ + H 2 O 2 Fe 3+ + OH + OH) to a toxic level, that ultimately, leads to a significant peroxidation in several cellular components (membranes, proteins, mitochondria, and DNA) (Schallreuter, 2014)

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Researchers measure downstream effects on NAD+ metabolite levels using LC-MS/MS methods and quantify lipid accumulation via fluorescent staining protocols

References Jastreboff, A.M., et al

J Biol Chem 273 : 35173527

For general use, one 300mg pill once per week is often suggested