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10 ul of each drug solution was added to 90 ul of the rat blood serum samples and at specific time intervals the researchers terminated metabolism by adding 1 ml of ACN and acetic acid in order to precipitate the proteins which were then removed after overnight refrigeration followed by centrifugation

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doi: 10.3390/nu7043011 70 AlipioM

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The primers (Sigma-Aldrich) used were: GLP-1R forward, 5-tctgcatcgtggtatccaaa-3 and GLP-1R reverse, 5-cttggcaagtctgcatttga-3