HeLa cells were treated with a series of concentrations of buthionine sulfoximine (BSO) for 72 h, a specific inhibitor for the enzyme required in the first step of GSH synthesis (Fig
Proc Natl Acad Sci U S A (1997) 94(24):1321520.10.1073/pnas.94.24.13215 255 RowlandJELichanskaAMKerrLMWhiteMdAnielloEMMaherSLet alIn vivo analysis of growth hormone receptor signaling domains and their associated transcripts
5.3.3 Improving Consumer Compliance and Repurchase Rates Without unpleasant odors and tastes, consumers are more willing to take the product long-term
An alternative procedure, using well plates and a plate reader, has also been described (Sauberlich, 1999)
In younger cells, FOXO4 appeared to be mostly in the cytoplasm, while in older cells it was more often in the nucleus, which may be considered its more active form
GAPDH expression itself was not altered under treatment condition (data not shown)