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Capsaicin demonstrates TRPV1-mediated miRNA modulation, with miR-449a significantly upregulated (34-fold) in prostate cancer cells, promoting androgen receptor degradation (4050% reduction) (102)

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then, the cells were washed three more times and stained in 50 l of PBS-B with goat anti-chickenAF488 and SAPE for 40 min at 4 C