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In this study, we carried a series of pharmacological studies of GLP-1R with daphnetin

Additional observed toxic mechanisms included loss of MMP, upregulation of the proapoptotic protein Bcl-2-associated X protein (BAX), and downregulation of antiapoptotic markers such as B-cell lymphoma 2 (Bcl-2), poly ADP ribose polymerase (PARP), and caspase-3 [134]

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After further culture of the cells they were resolved by sedimentation through 7% and 16% (w/v) iodixanol (800 g for 20 min